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To what extent each model will contribute to the full binding mechanism will depend on the required rate of binding, IDPs concentration, the native local plasticity of IDPs, the degree of binding degeneracy and the type of disorder-to-order transition.
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The change in the relationship of RK affinity for R* and the phosphorylation state of R* from an exponential one to a linear one required significant re-tuning of the basal rate of binding of Gt to R* (kG10).
The selectivity required for a suitable radiotracer is not fixed and depends on the relative densities of the desired versus off-target proteins, as well as the relative rate of binding of the radiotracer.
Hence, the increased rate of binding at higher temperatures.
The new agreement requires the use of binding arbitration.
To overcome this, the basal rate of Gt binding was required to be significantly faster.
"...increases the GTPase activity and the rate of nucleotide binding of several purified GTP-binding regulatory proteins (G proteins) whose function is to couple cell-surface receptors to intracellular mediators".
The glycan is required for binding of IgG to FcγR.
Yet despite the faster rate of activator binding, Brd2 recruitment is delayed (Table S2) indicating that it requires additional activator-induced regulatory steps to accumulate.
Rad52p is required for the binding of Rad51p to ssDNA.
Little or no activation energy may be required for this type of binding to occur.
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