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The basic requirements for setting up and scaling the bioprocess and the required purification strategy to obtain an economically feasible process yielding a product meeting the required specifications will be presented.
Moreover, the rapid access to water was required by beliefs and cultural traditions; various categories of Nages (uncleaned or untouchable) required purification and ablutions need to be performed before daily prayers (Ibrion et al. 2015b).
Note: Studies were conducted initially with the commercially prepared 20 kDa fragment of PA, from LIST Biological Laboratories, Inc., which required purification to remove minor amounts of contaminating PA63.
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After these activities have been completed, the priest stands outside the outer furrow, and the subject requiring purification advances to the first pit and is told to recite praises to the "Purity of Thought".
Development of acellular pertussis vaccine (aPV) requires purification of several components from Bordetella pertussis.
Likewise, CNTs are economically non-viable, difficult to produce at bulk scale and require purification, auxiliary treatment, and functionalization steps [10, 11].
With the yeast display method, on the other hand, HA can be prepared within several days and does not require purification.
At the same time, these approaches produce multi-shaped nanoparticles that require purification by differential centrifugation, and consequently have a low yield[12, 13].
The use of chitinases in chitin oligosaccharide preparation usually does not require purification of the enzyme, but chitinases in their purified form are required to study the biochemical properties, structure-function relationships, and their biotechnological applications.
Most of the current assays for proteasome inhibition are based on cell-free assays [18], [19], which require purification of 26S or 20S proteasomes from different sources.
Transgenic soybeans can also be used for production of therapeutic formulations that do not require purification.
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