Sentence examples for required origin from inspiring English sources

This TP-DNA might be generated using either a DNA that already contains the phage origins of replication (Fig. 1A, left side) or any linearized DNA after ligation of linkers containing the required origin sequences (Fig. 1A, right side).

The fork-shift model, which does not require origin relocation, is influenced by cis-elements and trans-factors associated with driving and maintaining replication forks.

This re-replication pathway is localized to the transposons and their flanking sequences and does not require origin re-initiation.

Methods for assessing network vulnerability found in the literature require origin-destination demands and path assignment.

Systematic improvement of experimental design requires origins of non-identifiability to be pinpointed and removed.

Finally, HAC vectors require origins of DNA replication that are functionally analogous to Autonomously Replicating Sequence (ARS) elements in yeast [ 17].

Prevention of origin firing by Rif1 would ensure that the limiting factors required for origin activation would be reserved for preferential use at early-firing Rif1-free origins.

Although FKH1 and FKH2 are not required for origin licensing, the binding of Cdc45 to early origins in G1 seems to be Fkh1/2-dependent, and it has been suggested that the role of Fkh1/2 in origin firing would involve the establishment of replication timing domains and the spatial organization of origins [ 11].

However, whether histone acetylation is required for origin selection or replication-timing specification remains an open question (10).

Under normal proliferative conditions, the activity of CDT1, which is required for origin licensing, is inhibited in G2 phase by interactions with Geminin (Klotz-Noack et al., 2012).

ORC1, a protein required for origin licensing, peaks in abundance in RO-3306 cells (4N DNA content), whereas in the elutriation data set, ORC1 peaks in elutriated cells with 2N DNA content.