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The collected trajectory data were gap filled if required (gaps were rare and typically only 2 3 frames wide, in very rare occurrences up to 10 frames) in QTM using NURBS interpolation and exported to Visual3D software (C-motion Inc., Kingston, Canada) where a Visual3D Hybrid Model for ideal rigid segments and 6-degrees-of-freedom (6DOF) was applied.
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Blending the mono-sized sands required gap grading and controlled packing density of fine aggregates (Goltermann and Johansen 1997; Park et al. 2004).
Further, to eliminate errors (Type I and II), we used the stringency of the minimum required gap, the threshold value, t for differentiating between species.
For assembly reasons, many hyperstatic mechanisms require gaps, which their functional requirements depend on.
A large number of microfluidic components such as cantilever beams, embedded channels and other shapes requiring gaps between layers are demonstrated in a single layer.
The W-curve does not require gap-stripped, reduced sequences to run efficiently.
The trinucleotide threading assay for analysis of STRs offers two levels of distinction: formation of a DNA thread requires gap bridging with a restricted nucleotide set followed by ligation.
Multiple sequence alignment did not require gap positions in any of the studied genes.
In addition to a PSSM, PSI-BLAST requires gap penalties as input: a gap opening cost and a gap extension cost.
Our results show that the Cx-dependent enhancement of apoptosis requires gap junctional communication and that this effect varies with the type of Cx expressed.
Together these data indicate that mural cell-mediated endothelial growth inhibition requires gap junction activity that is dependent on mural cell Cx43.
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