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An ITR which could be replicated by any wt or chimeric Rep was constructed by excluding the DNA elements required for specificity; the ITR5 spacer and the ITR2 nicking stem.
Further modifications would certainly be required for specificity.
The repetitive nature of HERVs makes designing assays that specifically detect individual elements difficult and carefully optimized probe assays are usually required for specificity [ 25].
SAM68 proteins have an hnRNP K homology domain (KH domain) that locates within a larger GSG (GRP33-SAM68-GLD1) domais that is required for specificity and high-affinity binding to RNA [ 7, 12].
Reducing the amount of supernatant required for specificity screening by microarray leaves enough hybridoma supernatant for further functional testing such as screening for the ability of the antibody to work in applications such as Western blotting, immunoprecipitation, immunohistochemistry, or other properties such as activating signalling pathways or blocking the binding of other proteins.
Similar(55)
This high percentage of E3 reflects the specificity that is required for specific substrate recognition.
ROOTMOTIFTAPOX1 sequence elements, which were required for organ specificity, were identified at the −251 position.
This work has identified those characteristics that are required for PC specificity and pH-dependent affinity for GALC.
Additionally, an improved understanding of the pharmacokinetics of these compounds may allow determination of the potential therapeutic index required for tumor specificity in vivo.
However, the precise number of SRK genes required for pollen specificity and the male and female determinants underlying SSI in the Asteraceae remains unknown.
This distinct interface is not required for the specificity of the binding but rather for stabilization, a mechanism that results in changing the structure of the histone fold domains of both CENP-A and H4.
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