The reason for this is not known but may reflect a requirement for S15 in a process required for replication of a damaged DNA template.
Ragland, R. et al. RNF4 and PLK1 are required for replication fork collapse in ATR-deficient cells.
The Center highly encourages researchers to archive their replication data, whether or not archiving is required for replication purposes.
Many of the mutations that lead to cancer are in genes that make proteins required for replication and maintenance of the genome.
We silenced twelve mammalian homologues by RNAi in HEK293T cells and identified three conserved factors, the PI4 kinase PI4KCA, the ubiquitin hydrolase USP22, and the ubiquitin ligase CDC27, which are also required for replication in human cells.
However, experimental evidence concerning whether autophagy is required for replication of coronaviruses has been contradictory.
Of the seven mutations that differentiate iMac239 from wild-type SIVmac239, a single change (D178G) in the V1/V2 region was sufficient to confer CD4 independence in cell-cell fusion assays, although other mutations were required for replication competence.
Further considering the fact that HCV propagation was reduced in siRBM24-transfected cells (Fig. 1), the inhibition of HCV IRES activity by RBM24 implies that RBM24 is probably required for replication of genomic RNA.
The early mRNAs encode proteins required for replication of the genome and transcription of intermediate stage mRNAs.
These observations indicate that the HA activity of the HN protein is not required for replication of APMVs.
Interestingly, about 50% of the bacterial loci required for replication in D. melanogaster-derived cells are also required for proliferation in adult fruit flies [23].
