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The type II system is the best understood of the three CRISPR systems, due largely to the fact that only one Cas protein, Cas9, is required for introducing DSBs [ 9].
Prior studies have found that external competition, influence of government policies, characteristics of target audience, and source of funds required for introducing new IS [ 42, 54, 55] are key factors for successful IT implementation.
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The CP together with the signalling messages required for synchronization introduce a lot of overhead, reducing the spectral efficiency.
The SAR analysis revealed that (i) the carboxyl group at the 11-position was required for activity; (ii) introducing of a substituent on the nitrogen atom at the 12-position of 3, especially substituted benzyl, might significantly improve the activity.
We remark that to establish the viscosity theory certain semi-regularity is required for semisolutions, as introduced in (Ekren et al. 2016a; 2016a).
They were there for a press conference, because later that day councilman Rafael Espinal chair of the committee on Consumer Affairs would be conducting the first-ever city council oversight hearing on the Cabaret Law, a formal step required for Espinal to introduce legislation to repeal it.
To identify key residues required for function, we introduced mutations into the FATC domain.
The energy required for dispersing is introduced by mechanical agitation or sonication.
The G to S and R to Q double mutation in the Boo BH1 framework removes a principal ionic interaction with BH3-only proteins required for binding and introduces the polar hydroxyl group of serine into the hydrophobic groove.
Three major goals are pursued: firstly, reduction of human resources required for data management by introducing automated processes.
We now dispose of both the parameter values and the accompanying sufficient statistics, which are required for the bootstrap algorithm introduced in Section 3.2.3.
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