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This repeat is preceded by a conserved A/T rich sequence that is required for function.
ATP hydrolysis by Rvb1/2 is required for function; however, the mechanism that drives substrate remodeling is unknown.
Individual and combined joint movements required for function such as activities of daily living are discussed, including normal arthrokinematics and the components that contribute to joint stability.
Par-6 PDZ regulation is required for function as mutational disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in polarized MDCK epithelial cells.
It is demonstrated that in the commonly encountered cases where a discontinuity resides on a small subset of the dimensions, the present method becomes "optimal", in the sense that the total number of points required for function evaluations depends linearly on the dimensionality of the space.
As validation of this system, we introduced three copies of tetO into the promoter region upstream of the cagUT operon (encoding two virulence factors required for function of the H. pylori Cag type IV secretion system) and expressed tetR by introducing a codon-optimized gene into the chromosomal ureA locus.
The de novo design of artificial metalloproteins from first-principles is a powerful strategy with which to establish the minimum structure required for function, as well as to identify the important design features for tuning the chemistry of the coordinated metal ion.
If the C-terminus of a protein is required for function then this will not be an appropriate method.
We show that all three known type II BMP receptors are expressed by WEHI 274.1 cells but that only ActRIIA and BMPRII are required for function.
General understanding of the mechanisms required for function at origins of replication has grown immensely since the first prokaryotic origins were characterized.
Our studies clearly indicate that Ppt1 protein is expressed ubiquitously at a very low level such that only a minute amount is required for function.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com