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It is important to note that the percentage of hub neurons required for disruption here was clearly larger than the 1% needed to disrupt structures in clusters at the 6 neuron scale.
After purification, homologous recombination at the cre2 locus, required for disruption of the ORF, was verified using Southern analysis.
Despite the consistency in results between models, the H. pylori virulence factor required for disruption of occludin remains to be determined.
Our finding that similar concentrations of NDGA are required for disruption of actin stress fibres, JNK activation and the induction of apoptosis further supports our conclusion that these events could be related.
Targeting of Listeria during intercellular infection was, however, found to depend on the bacterium's phospholipases A and B; these activities are required for disruption of the double vacuolar membrane resulting from both donor and acceptor cells (Birmingham et al., 2007; Py et al., 2007).
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D1 receptor homooligomers could also be disrupted by agonist, but at higher concentrations than that required for the disruption of the D1 D2 heteromer.
As high pressure is required for droplet disruption into the submicron range high investment, energy and maintenance costs result from high pressure homogenization processes.
However Mdm2 does not appear to be required for PML disruption by USP7, as Mdm2 silencing had no noticeable effect on the ability of overexpressed USP7 to induce the loss of PML NBs (data not shown).
MPO and ROS are required for extracellular disruption of IAV [ 20].
Therefore, the H297 and K314 residues are not required for the disruption of silencing observed when Epe1 is overexpressed.
To produce a homozygous cell clone, two selectable markers are normally required for the disruption of both alleles, and one further selectable marker is needed for phenotype complementation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com