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The rates of complementation and the times required for colony formation are summarized in Table 1.
These data suggested that the VEGF autocrine loop is required for colony formation in SS.
(A ) ATM is not required for colony growth in soft agar.
In all clinical Sporothrix spp. evaluated, we found significant differences between the minimum concentrations needed to inhibit fungal cell growth and the concentration required for colony count reduction.
Furthermore, after combined expression of c-MYC and mutant RAS in MEFs, AP4 was required for colony formation, anchorage-independent growth and tumor formation in mice.
We conclude that the Rep helicase (and not UvrD) is required for colony formation on LB when at least three highly transcribed rrn operons are oriented opposite to replication.
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Efficiency in synthesis of cytoskeleton proteins is required for tumor colony formation, partly explaining why treatment with F4 cells cannot form colonies in soft agar.
The minimum bacterial inoculum required for L-form colony formation was approximately 104 105 bacterial cells, which is consistent with the finding reported previously in the literature [12].
Four mutants, rcsB, a response regulator of colanic acid capsule synthesis, ruvA, a recombinational junction binding protein, fur, a ferric uptake regulator, and smpA, a small membrane lipoprotein, were chosen for complementation studies and all were found to be required for L-form colony formation.
We determined the degree to which Taspase1 is required for the colony formation capability of HER2-positive breast cancer cells on soft agar.
As we previously reported that JAG2 expression was required for spontaneous colony formation, we monitored JAG2 expression following inhibition of PI3K/AKT pathway by IGF1R silencing, imatinib and wortmannin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com