Sentence examples for required final concentration from inspiring English sources

Aliquots were taken from these suspensions and further diluted with culture medium until the required final concentration was obtained.

Cells in growth phase were detached, suspended in medium, diluted to double the required final concentration, and maintained at 37°C.

An aseptic rMnSOD solution was prepared separately in water for injection and sterilized by Millipore 0.22  μm filters; it was added to the gel formulation to give the required final concentration of 2.0  μg/mL.

Both peptides were dissolved in 10 mM NaOH to avoid aggregation and then diluted into phosphate buffered saline (PBS 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and 2 mM KH2PO4) 10 mM PBS, pH 7.4 to the required final concentration, determined by Bradford assay and by absorbance at 220 nm [ 2].

Compounds (133 × final concentration in DMSO) were diluted into Refolding buffer before the addition of NS2 3 to yield required final concentrations.

Schneider's Drosophila Medium (revised) (Gibco), containing 5% fetal calf serum (Gibco), was spiked with the respective stock solution to achieve the required final concentrations.

For root assays, different concentrations of filter-sterilized methyl-JA and SA solutions were added to sterilized HL medium to obtain the required final concentrations and seeds were plated on it [ 45].

Then, 450  μl of the 2.3  μ M glycolipid solution was immediately diluted into 50 ml of warm BHI to give the required final concentrations of 20 n M glycolipid and 0.05% tyloxapol, and this was inoculated with 500  μl of a LM-Mb mid-log phase culture (OD600 0.5 0.8).

Saliva sample containing Δ9-THC was prepared by firstly dispensing a known volume of 10 ug/mL Δ9-THC/methanol into a glass vial evaporating the methanol, then adding a known volume of saliva to achieve the required final THC concentration.

Antibiotics were added to media when required at final concentrations of 50 μg/mL of kanamycin for both Xoo and E. coli; and at 100 μg/mL of ampicillin for E. coli.

These will still require final Vatican approval.