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We realize that in some cases larger volumes are required due to sampling statistics but, overall, microfluidics has proven to be the next step in the separation of small volumes.
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Judged by pupil self-assessment forms, our preliminary trial was a success, though caution is required due to the small sample size.
The self-reported ancestral and genetic structure data lend evidence to a genetic contribution of the region of the current Czech Republic to New Hampshire despite the lack of historical record, though more study on this topic is required due to the very small sample size of the Czech group.
A limitation of this study was the use of blinded samples, required due to the nature of the funding used, wherein the clinical information from the patients was not available.
A great advantage of the developed method is that no sample pretreatment is required due to an online extraction method based on a six-valve system allowing a sample volume up to 100 mL [ 53].
Using rule of thumb of 10 participants for each variable (Harrell, 2001) and inflating the sample size required due to the presence of repeated measurements by 1.32, 160 participants are required to estimate up to 12 coefficients in a model.
Samples from routine TB patients were used to reduce delays that would have been required due to approvals and consenting processes if patients had been approached.
Validation of the model should be conducted on an independent sample rather than a subset as required here due to sample size.
This is due to sample size limitations and incomplete information and requires efforts for a large-scale follow-up.
Due to sample size and inconsistent reporting of specific specialties, we were required to combine several specialties for statistical analysis purposes.
This suggests that adjuvant chemotherapy did not provide survival benefits to subtype V patients in the early stages; however, this would require further confirmation due to small sample size.
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