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Homogeneous radiation protective materials of the Abris type were used in the studies, the manufacturing technology for which makes it possible to obtain the required concentrations of filling agents.
HT-29 SN-38R and HT-29 FUR cells required concentrations of SN-38 or FU about 10 times higher than wild-type HT-29 cells to exhibit similar rates of cytotoxicity or apoptosis (data not shown).
This suggests a Hill-type model, U pd = U i (EAA j ; V pd, β a, ), with being the minimum required concentrations of the j ∈ [1, 2,..., 20] intracellular EAAs (given, for instance, by [ 35, 43]).
A melphalan concentration as low as 33.9±12.2 μg ml−1 was sufficient to induce detectable levels of apoptosis in responders, whereas non-responders required concentrations of 61.7±14.0 μg ml−1.
Specifically, BMPCs from responders showed evidence of p53 phosphorylation at melphalan concentrations as low as 45.2±11.7 μg ml−1, whereas non-responders required concentrations of 70.8±13.2 μg ml−1 (Table 2).
The required concentrations of each essential and beneficial element vary over a wide range.
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This is well above the minimum required concentration of dissolved oxygen for optimal fish habitation.
Apomorphine HCl (Apo, Sigma Chemicals, St . Louis USA) was dissolved by sonication in sterile 0.9% NaCl solution to obtain the required concentration of 2.0 mg/kg.
Haloperidol (Hal, Janssen-Cilag, Baar, Switzerland) was prepared from solvent ampoules (5 mg/ml) diluted with sterile 0.9% NaCl solution to obtain the required concentration of 0.4 mg/kg.
From this required concentration of extract was taken by sterilized pipette in a sterilized petriplate and then 15 ml medium was poured into the petriplate and mixed well and allowed to solidify.
The Department of Energy estimates that practical hydrogen storage will require concentrations of at least 6.5percentt.
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