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Pab87 (7 mg mL−1) was mixed with lanthanide complex solution at required concentration to obtain Lu-HPDO3A final concentrations of 50, 100 and 200 mM.
For inhibition, test reagents were added to reach the required concentration to the well.
These solutions were mixed and diluted to the required concentration to give a standard solution.
Despite the above, previous generation of GGTIs demonstrated significant toxicity when combined with FTI at the required concentration to achieve loss of KRAS prenylation [ 26].
The required concentration to inhibit the cell growth by 50% (IC50) for aqueous extracts of G. lucidum, G. neo- japonicum and G. frondosa were 1298.71 ug/ml, 3037.32 ug/ml and 4384.68 ug/ml, respectively.
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Values into brackets in front of KH2PO4 and K2HPO4 indicate the required concentrations to obtain a buffered media for pre-cultures To study isoleucine starvation response, L. lactis was grown in batch cultures in similar medium (Table 1).
Each fraction was solvent evaporated in a rotary evaporator (40°C), and the residue weighed before being dissolved in DMSO to the required concentrations to assay for antiproliferative activity as detailed below.
Tokyo's subway system requires concentration to navigate (with the help of an English-language map), but it's affordable.
To make a turntable that draws rave reviews requires concentration; to hear a sound thinning from a long-playing record as the needle is raised one-thousandth of an inch requires an understanding of the many shades of quiet.
The water is dirty and opaque, so each step requires concentration, to make sure that you have sure footing and aren't stepping in, say, a sewage drain that's missing its manhole cover.
"It's a highly charged, moving play, which requires concentration to watch as well as real sensitivity from the performers.
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