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For highly polydisperse systems this leads to an effective lowering of the minimum required concentration for successful fibre spinning (in comparison to narrowly distributed polymer solutions of similar weight average molecular weights).
In addition, the concentration of acid/oxidizing reagents needed to complete the acid leaching (2.5 M, 3.0 M and 0.5 % (m/v) for nitric acid, hydrochloric acid and hydrogen peroxide, respectively) is lower than the required concentration for a conventional microwave-assisted acid digestion (concentrated acids).
We note, however, that 1269 is considerably more potent than 1245, therefore the required concentration for TBV activity for 1269 alone is much lower than for a 1245/1269 mixture.
Detection of prion proteins has achieved the required concentration for clinical diagnosis in blood serum, which is less than 2 nM.
After the PS fiber was separated from the alkaline liquid, it was resuspended in appropriate HCl solution of the required concentration for neutralization of both residual NaON and CaCO3 contained in PS and soaked overnight.
The synthetic wastewater solutions were prepared in double distilled water (pH 6.5 6.7) using Pb NO3 2 (from Panreac); the dilute solutions at the required concentration for experiments were prepared from initial solution at 1,000 mg/L of Pb II).
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The extracts were redissolved in their respective solvents to the required concentrations for the bioassay analysis.
Solutions of docetaxel dissolved in DMSO at 100 μM and 150 μM were used to obtain the desired final required concentrations for IC50 and 1.5× IC50.
Peptides were subsequently dissolved in DMSO to a 10-mM concentration, followed by the addition of deionized water to 1-mM concentration, and further dilution into culture media to the required concentrations for the different experiments.
Low concentrations of NO protect cultured keratinocytes and skin from oxidative stress and UVA-induced apoptosis.,, The mechanism and the required concentrations for this protective action in skin are still unknown.
A stock solution (2 mg/mL) of each compound was prepared and then diluted in RPMI 1640 (Sigma-Aldrich Co., St . Louis MO, USA) to the required concentrations for the assays.
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