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To obtain the required cell number for flow cytometry analysis, 15 mL of culture infected with either bacVP6, bacGFPVP2 or co-infected were centrifuged at 1,000× g for 10 min in an Eppendorf 5810R centrifuge (Hamburg, Germany) at 4 °C degrees.
Cell proliferation is one means of attaining the required cell number.
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To deliver the required cell numbers and doses to therapy, scaling-up production and purification processes (at least to the liter-scale) while maintaining cells' characteristics is compulsory.
To achieve required cell numbers and to reduce process-related risks such as cell transformation, relative short batch-like production processes at industry- and clinically-relevant scale(s) must be developed and optimized.
Transferring established screening protocols from immortalized cell lines to primary cells is not trivial and technical bottlenecks include required cell numbers, susceptibility to small interfering RNA (siRNA) transfection, assay robustness and the issue of heterogeneity accompanying primary cells [ 17].
Research into development, disease, and the effects of drugs require cell number counts.
Of course, since about half of American households now only use cell phones, pollsters have to include cell phones in the sample, despite the fact that federal regulations require cell numbers to be dialed manually, rather than with an autodialer, dramatically increasing the cost of calling.
The Registrar is very serious about requiring cell phone numbers and contact info.
Examination of the resolution interval (Ri; defined as the time required for cell numbers to reach 50% of the numbers observed at the peak of inflammation (Bannenberg et al, 2005)) of total inflammatory cells in the BALF revealed a shift from 4.9 days for wild-type mice to 2.9 days for R-848-treated mice and 6.1 days for Tlr7 −/− mice (Fig 2B).
The methodology presented herein provides a number of advantages with regard to the monitoring of protein function: to date such analyses were usually carried out as end point analyses on cellular material that was previously destroyed by detection markers or fixation procedures and which usually required high cell numbers while yielding unsatisfactory information on cellular response kinetics.
(1) The concentrations of ETRB antagonists required to reduce cell number are far above the concentrations required to displace ET-1 from ETRB.
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