In fact, polymerase pausing has not been observed under the conditions used here for a reporter that is without chromatin structure and introns and does not require synchronized expression during development and differentiation.
For example, electrogenic release of Ca2+ from and reuptake of Ca2+ into the ER/SR require synchronized counterion movements to balance the buildup of charge asymmetry across the ER/SR membrane, yet the molecular machinery responsible for the proposed counterion fluxes remains undefined.
The mammalian system has a linear flow (eye hypothalamus pineal gland) to the rhythmic production of melatonin, whereas in fish, melatonin biosynthesis and degradation require synchronized complex interactions among eye, pineal gland, brain (particularly hypothalamus and pituitary), and peripheral tissues upon the stimulation with light (Falcón et al. 2010).
Thus, PDT requires synchronizing interactions among light, photosensitizers, and oxygen to be effective.
To account for staggered entry requires synchronizing infectious challenge in the statistical model to calendar time, rather than time since entry into the study.
Connecting map positions to GO terms requires synchronizing several information sources, including livestock maps, human sequence annotated GO terms, and GO databases.
Formation of the heart tube requires synchronized migration of endocardial and myocardial precursors.
Reconfiguration in response to a fault [54], or predictive reconfiguration in response to an anticipated damage or loss of generation, requires synchronized measurements and millisecond decision making and communications to the local actuators.
However, comparing the embryonic transcriptomes produced during sexual and asexual oogenesis requires synchronized embryo development.
We speculate that a complex splicing event is rather an integrated "module" that requires synchronized changes in neighboring exons, than merely a simple ASE accidentally coupled with exon extension/truncation events.
