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To verify the potential usefulness of such thermal models would require probing the temperatures at depth at specific locations matching that of geotherms presented here.
Further complications of the detection scheme in force-based SPMs require probing of the response in a frequency band around resonance (since resonant frequency can be position dependent and single-frequency methods fail to capture these changes) [29-32].
Autofluorescence can interfere with techniques that require probing such as microfluidic FACS.
Going beyond a specific cognitive domain, such as vision or pain, and studying functional specialization in a broad sense require probing more functions than can be addressed in one experimental paradigm.
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Deep veins require probes of around 6 MHz whilst the abdominal vessels are better studied with probes of between 4 and 6 MHz.
However, both PET and fluorescence imaging usually require probe labeling and moreover are low in spatial resolution.
This may be particularly significant when designing probes for transcript variants where alternative exons require probes distal to the end of the gene transcript.
Among them, the SYBR Green I dye method does not require probe labeling and is highly cost effective and therefore has been more widely used.
For example, using a stringent 3 consecutive probe criteria, deletions of ~100 bp require microarrays with probes staggered ≤ 45 bp while smaller deletions of ~30 bp need probes staggered ≤ 32 bp and deletions of a just a few bp require probes staggered ≤ 12 bp.
Splicing arrays exist but they require probes designed to be complementary to junctions, and these can therefore be generated only if the genes and the distinct isoforms produced from them are already known [ 35].
Ensuring the beam quality is maintained for such applications requires probing of the electron beam itself, and not just monitoring of process parameters.
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