The formation of periodic layered structure requires an incubation period t1, while if the immersion time is over t2, the periodic layered structure is destroyed.
In addition, this enzyme can be heat-inactivated and requires an incubation temperature of 37°C in contrast to the highly stable Thermotoga maritima enzyme.
Optimal in vivo antibody staining with a good correlation with ex vivo staining required an incubation time of approximately 1 h for the antibodies to reach dynamic equilibrium following egress from the vasculature and accessibility of the target cells.
Detecting wound healing at this resolution required an incubation time of 24 hours after wounding as differences are not readily seen with this method at the 7 hr timepoint.
Even more importantly, if a study involves repair kinetics, i.e. requires an incubation of cells for certain time periods after irradiation under normal growth conditions, irradiation for an initial t = 0 and subsequent time points should be carried out in an identical way.
Identification of isolates may require an extended incubation period (days), delaying laboratory reports and indirectly affecting therapy options and treatment.
In this model, mycobacteria are grown in stirred, airtight tubes with a defined headspace and require an extended incubation period to consume the headspace oxygen, enter hypoxia, and cease replication.
Biochemical and culture-based assays are common detection methods, but require a long incubation time and easily misidentify Y. enterocolitica as other non-pathogenic Yersinia species.
A third supply of 10 mg/L TTri and BTri at day 77 was performed to test whether 4-TTri might require a longer incubation to be removed.
Other mechanisms of laminin-surface interaction may be occurring during this process in order for cell attachment to require a minimum incubation time of 12 h.
