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A selection coefficient of s = 10−3 and a mutation rate of 10−8 per site, for example, would require a locus of length 10 kb to achieve u/ s = 0.1.
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Moreover, traditional enrichment-based approaches for isolating microsatellite loci require a priori choices about what types of microsatellite loci to target (both repeat size, and repeat motif sequence) which will ultimately lead to limited success in obtaining sufficient numbers of different types of useful microsatellite loci [ 26].
The identification of respective candidate loci, however, would require a genome wide scan with numerous loci.
This would presumably require a greater number of loci [ 52].
Generally, informative VNTR loci require a good balance between variability and stability.
Studies to identify phenotypically-associated polymorphisms in the Plasmodium falciparum 23 Mb genome will require a dense array of marker loci.
It should be noted that the high allocation power in our analysis might be due to the limited number of parent pairs, and that the assignment of a progeny to a larger parental stock might require a higher number of marker loci.
For example, a trait which is controlled by s =10k loci would require a sample size of n ∼300k individuals to determine the (linear) genetic architecture (i.e., to determine the full support, or subspace of nonzero effects, of x ).
The second uses PCR to attach an MID (Binladen et al. 2007) but is exceptionally expensive because it requires the purchase of long primers (35 55 bp, depending on application) for every combination of primer and barcode to match each sample used in the study (e.g., 12 samples × 12 loci would require a total of 288 primer pairs).
Populations with different histories may harbor different levels of polymorphism and this might affect estimation of the transition length required for a locus to become a microsatellite.
(Response) The p-value and log2 fold-change required by a locus' response in order to qualify for the respective class.
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