Thus, two of the referees would like to request experiments to reproduce some of the anti-34a experiments reported in the original study, such as Figure 1J.
If a requested experiment is easy to do, just do it, even if it doesn't tell you anything you did not already know.
We show that all the currents set in all optical elements of the simulated column can be implemented in the real column (I2TEM in CEMES) and used as starting alignment for the requested experiment.
The requested experiment has been done.
We are working on this question, but cannot yet do the requested experiment.
As detailed below, the requested experiment 3 does not address the issue, However, we have now done a set of in vitro experiments that does.
The requested experiment is impossible to perform because the primary normal and many cancerous mammary cells fail to form organoids in the absence of Wnt agonists.
In particular we did the requested experiment, in which we seeded Shine-Dalgarno sites in three genes, right in the place where it often naturally appears.
Without translation, Rho rapidly terminates transcription in vivo and the only feasible way to conduct the requested experiment is to inhibit Rho with bicyclomcin (see Ederth et al., 2006).
Additionally, to make complete the requested experiment we must be able to measure elongation rates when H NS filaments form on the bgl DNA, including when they are stabilized at 20°C.
I understand the difficulties the authors had to address the major concern #2 and I think that, even when the requested experiment would improve the manuscript its absence doesn't make the manuscript any weaker.
