Primers used (sequence available from the authors upon request) amplify a 101 bp region in the 3' end of the 3'UTR.
He refused a defense lawyer's request to amplify his response.
Two sets of primers were designed (Invitrogen; http://www.invitrogen.com, sequences available upon request) to amplify 2 promoter regions, 112,621,625 to 112,622,006 and 112,622,164 to 112,622,604 (NCBI Build 36.1).
Interscope executives did not respond to requests to amplify on the label's relationship with the band.
Those requests were repeated and amplified as ISIS militants seized Mosul, Iraq's main northern city, on Tuesday and began moving southward.
Furthermore, we designed splicing-specific and skipping-specific primers (sequence available on request) to selectively amplify abnormal transcripts predicted by bioinformatic tools, even when this was not detectable on RT-PCR.
The orchestration over all is rich and thick, so following the composer's request, the solo violin was lightly amplified for proper balance.
The Henry plays have often been appropriated as conveyances for political messages: when Laurence Olivier directed and performed the lead role in a film version of "Henry V," in 1944, British government officials requested that its patriotic themes be amplified.
To confirm that each transgenic line contained fosmid DNA, a 600 bp PCR product targeting the fosmid vector sequence was amplified (Primer sequences available upon request).
Therefore, all exons were separately amplified using PCR (primers available on request) as described previously by Sidransky et al (1991) and modified by Van Houten et al (2000).
