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While we observed Groucho-mediated repression, the effects were much more variable than with HMG-based proteins (data not shown).
To identify whether inhibition of CCND3, just like miR-138 restoration, also resulted in HCC repression, the effects of knockdown of CCND3 on cell growth were examined.
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As shown in Fig. 6G and H and Fig. S4D and E, Atg7 repression resembled the effects of Atg5 repression in these cells.
The effects of CreB on carbon catabolite repression and the effects on the regulation of permeases are proposed to operate via separate mechanisms, and thus the complementation of the range of phenotypes indicates that both functions are conserved between the two orthologues.
This could lead to a relief from a potential repression, mimicking the effect of its overexpression [ 17].
This phenomenon is termed nitrogen catabolite repression, although the effect is not as well characterised as its carbon counterpart, particularly with respect to sudden changes in ammonium availability.
To explore the role of eIF4E2 in miRNA-mediated translational repression, we analyzed the effects of eIF4E2 downregulation on miRNA silencing of two reporters, the Let7a-responsive LRE reporter and the miR-19-responsive 19RE reporter (Fig. 3A).
To further demonstrate the role of eIF4E2 in miRNA-mediated translational repression, we analyzed the effects of eIF4E2 downregulation on the expression of endogenous IMP1 in HeLa cells wherein let-7a is highly expressed.
To further establish a causal relationship for the CRK and SP1 interaction in p120ctn transcriptional repression, we examined the effects of concurrent SP1 and CRK manipulation on the p120ctn expression.
In conclusion, the transcriptome of Nongken 58S was significantly suppressed and the repression effects were markedly intensified when inflorescence development proceeded.
Some portion of the C-terminal domain may be important for mediating the repression effects of sRNAs, and one model proposes that these domains may help to recruit the Hfq:sRNA complex [74,75].
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