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This repression did not rely upon a functional HCL gene but was LIN-dependent, thus defining a new repression pattern termed R3.
Finally, a single gene from the NN class possessed a distinct repression pattern (R4) which was unaffected by any of the tested mutations.
This differential repression pattern is exactly what we found for INK4-ARF in hESCs and KRAS-positive CRC cells.
Using the MK-ER-Ndt80 strain, we were able to quantify the effects of Sum1's binding to NDT80 on the repression pattern in late meiosis phase.
The ratio between the relative expression values at late and middle meiosis were calculated for the 140 Ndtargetrgenesenes that show late meiosis repression pattern.
A highly consistent DNA hypermethylation and mRNA repression pattern was obtained for both GRASP (69%) and TSPYL5 (73%), suggesting that their biological function is regulated by DNA methylation.
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However, the repression patterns were variable, suggesting the existence of several repression pathways.
Two repression patterns were observed amongst these genes (Table 5, Table S6, Figure 7).
A looping back model have been suggested previously to explain the repression patterns observed at native telomeres[6].
The use of testes at various developmental stages highlighted several gene activation or repression patterns throughout the reproductive cycle.
Many of the genes included in a SOM group showing evident induction or repression patterns were not detected as being differentially expressed according to the outliers searching method.
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