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Secondly, the interpretation of transcriptome data obtained from the generally employed shake-flask cultivations is likely to be complicated by differences in specific growth rate, carbon catabolite repression, nitrogen catabolite repression, and more generally continuous changes in environmental conditions.
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BAT1 expression was not influenced by the quality of the nitrogen source, and VIL repression was observed on either repressive or non-repressive nitrogen sources (Figure 3B and 3C).
In contrast to the transcriptomic repression of nitrogen assimilation, 35 contigs/singletons related to protein turnover processes were induced in response to copper stress.
The down-regulation of scfA on suberin (Table 4, AN1303, which encodes a similar transcription factor and its deletion leads to farB deleted mutant phenotype [ 22]) might be explained by its repression under nitrogen limiting conditions [ 64].
Although the regulatory cascades that control the transcription of the nif genes in proteobacteria have been well investigated, there are limited data on the kinetics of ammonium-dependent repression of nitrogen fixation.
A second regulatory loop, involving OAS as a key intermediate, should act in promoting gene de-repression when nitrogen and carbon supply exceeds sulfur availability within the cells.
Relief of nitrogen repression at the transcriptional level due to absence of nitrogen-rich sources is called nitrogen catabolite repression (NCR) (Hofman-Bang 1999).
A larger change in the magnitude of expression of differentially transcribed genes was observed in response to carbon catabolite repression than to nitrogen catabolite repression.
The prototype of this family is NmrA, a negative transcriptional regulator involved in metabolite (for example nitrogen) repression in various fungi, including N. crassa and A. nidulans [ 59].
Moreover, yeast exhibit nitrogen catabolite repression (NCR) in which preferred nitrogen sources repress expression of genes required for uptake and catabolism of less preferred nitrogen sources (Magasanik and Kaiser 2002).
The regulation of AaSP2 transcription was not controlled by nitrogen catabolic repression.
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