Sentence examples for repression improved from inspiring English sources

Although methylation of either of the downstream or upstream region was sufficient for transcriptional repression, improved response to treatment as measured by PFS was observed only for Group C and Group D where a larger part of the promoter region was methylated.

Similarly NF-κB is activated during brain ischemia and its partial repression improves the damage [ 31].

Primers used to produce these constructs are described in Supporting Information Table 3. LacI mediated repression was improved by replacing the PlacIQ promoter in pACSA_PcptOO_YFP_PlacIQ_ lacI with members of the synthetic promoter library using Gibson Assembly (see Supporting Information Table 3 for primers used).

LacI repression was further improved by making a W220F point mutation via Quikchange site directed mutagenesis.

In an important study, the tethering of a silencer-flanked reporter construct near telomeric foci was found to improve repression in an SIR-dependent manner (Andrulis et al, 1998).

In a recent study, Lohmueller et al. (80) fused various leucine zipper (LZ) homodimerization domains to ZF-TFs and found that these added domains improved activation and repression up to 2.5- and 7.5-fold, respectively, using a human c-Jun LZ (dimerization kd = 448 μM) and up to 10-fold and 8-fold, respectively, using a stronger homodimerizing yeast GCN4 LZ (kd = 8 nM).

A spontaneous mutant AFP111 (NZN111 ptsG −) has also been shown to exhibit cell growth on glucose and improved anaerobic succinate production with suppressed normal glucose repression (Chatterjee et al. 2001).

Other examples where chemical analogs can be used to select phenotypically improved variants are approaches to select cells lacking catabolite repression.

For this circuit the mechanism is well understood: an excessive increase of the repression strength (while potentially improving the noise reduction of the circuit) reduces the copy number of mRNAs and proteins with a consequent rise in intrinsic fluctuations (which can overcome attenuation).

This plasmid harbors an improved copy of the tetO promoter in pALC2073 that ensures complete repression when non-induced and high expression when induced by anhydrotetracycline.

Overexpression of the GmPHD2 improved salt tolerance of the transgenic plants, indicating that proteins with transcriptional repression can also confer stress tolerance.