In agreement with these data, addition of glucose to shp1Δ and rts1Δ pph21Δ pph22Δ strains resulted in a 6-fold higher residual phosphorylation compared to WT repressed levels.
A recent study indicates that pluripotency factors repress levels of Rnf12 [ 30], although set against this, ectopic expression of Rnf12 in ES cells with the intron 1 NOS deletion does not trigger Xist upregulation [ 23].
daf- 2 mutants also displayed repressed levels of protein synthesis using S methionine incorporation (Depuydt et al. 2013), although this was not seen in a previous study (Hansen et al. 2007).
In the wild-type strain, where FLO1 transcription is repressed, levels of acetylated histone H4 (H4and acetylatedated lysine 9 of histone H3 (H3K9ac) were low over the entire 3.5 kb FLO1 promoter and upstream region (Fig. 4C and D, wt).
After 2 hr of treatment, all of the compounds decreased the levels of the 2,751 probes in pattern 16; however, after 24 hr, only DES and E2 maintained the repressed levels of the probes, but the short-acting estrogens returned to the vehicle level.
However, the residual phosphorylation of Snf1 (6-fold higher than the WT repressed level, but lower than the WT derepressed level) appears to be enough to sustain the highly elevated expression of SUC2 in the derepressed condition.
To confirm that activation of PERK-eIF2α-P signalling is indeed the result of mutant tau expression in rTg4510 tauP301L+ mice, we tested the effect of repressing tau levels on eIF2α-P and ATF4 levels using doxycycline administration.
This latter TF was more efficient in repressing the levels of the basal marker KRT14 before and after differentiation.
