We measured the levels of ERα protein by Western blot analysis in the different clones and investigated whether the effects of unliganded ERα were activating and whether the effects of E2-bound-ERα were repressing based on the levels of ERα.
We eventually selected activating or repressing candidates based on their co-expression with or adjacent to rx2 in the juvenile CMZ.
Among the genres repressed was music based on tindé drums, which Mr. Morgan describes as "the mitochondrial DNA of all Tuareg music".
The putative Arabidopsis orthologs that are induced or repressed by cold, based on ColdArrayDB analyses are shown in Table 2.
Interestingly, it was shown previously that RUNX1 is a direct target of ELF5 and is repressed by it, based on chromatin immunoprecipitation (ChIP) analysis (Kalyuga et al., 2012).
From the profiling data, we filtered and identified the top miR-184 repressed mRNA targets based on their fold change (Table 2).
We looked in the target list for genes that could be related to N metabolism or to root growth regulation and that were either induced or repressed by nitrate based on our Illumina results.
To further characterize the role of the miRNAs in response to cold, we examined the target list for genes that could be related to the cold response and that were either induced or repressed by cold, based on our Illumina results (Additional file 17).
In this model, estradiol selectively enhances or represses gene expression based on the dominant ER isoform expression and profile of cofactors present in the cell.
MiRNAs recognize and repress target mRNAs based on sequence complementarity, and are critical in regulating a variety of biological processes, including cell cycle, differentiation, development, and metabolism, as well as such diseases as diabetes, immuno- or neurodegenerative disorders, and cancer [ 8].
