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The mtDNA copy number was calculated using the following formula: [ X μg/ μL plasmid DNA/4419 (plasmid length) × 660] × 6.022 × 10 = Y molecules/ μL, where X represents the concentration of plasmid DNA and Y represents copy number.
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Microsoft's 40 million figure, in contrast, represents copies of Windows that Microsoft sells to all of its customers.
Complications may also arise if polymorphisms are discovered through transcriptome analysis, since the transcriptome only represents copies of expressed alleles.
Figure 2B shows the results from nine CNV states (as columns), representing copy number 0 through 4, mixed with four different proportions (as rows) of normal cells.
Using a HMM with up to 20 states representing copy numbers from 0 through 7, we developed a novel computational framework (MixHMM) for detecting copy number and allelic imbalance accurately.
For example, ratios of one and two represent copy number two and four respectively.
Let the observations representing copy numbers in p genomic loci be y= y1,…, y p ), ordered according to genomic position.
The amount of KDR mRNA was normalized to 106 copies of the β-actin internal control, and the data represent copy number/106 β-actin copies.
F r1) gives a measure of how distant the determined copy numbers are from integer numbers, if r1 is chosen as representing copy number 1.
Regions identified with variable depth of coverage between pools of dog breeds from the SC data that could potentially represent copy number variation.
In the present study, 36% of canine HS cases demonstrated CNA of TP53, representing copy number loss in 9.3% cases and gain in 26.7% of cases.
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