The difference between the dominant MLVA types representing isolates with SpaTypes t011 and t108 is caused solely by the presence of an extra spa repeat (r34) in t011.
The approach was investigated using 47 C. jejuni isolates, previously characterized by classical MLST, representing isolates from diverse environmental, animal and clinical sources and including the six most prevalent sequence types (ST) and the most frequent alleles.
All Chinese Echo7 strains fell into subgroups B1 and B2, and group C, representing isolates from 1998-2012, 2009-2010, and 1998-2010, respectively.
Since 1988, the HA- and NA-producing genes of avian influenza subtype H7N9 have been deposited in GenBank 12 times, mainly representing isolates collected from wild bird hosts (Table).
The same comparison with any reference, regardless of annotation, yielded values of 445 (66.1%), 165 (24.5%), and 61 (9.1%) isolates, with the latter group representing isolates highly divergent from any previously sequenced organisms.
All strains from Shandong, together with 5 strains from the Chinese provinces of Guangdong, Jilin, Anhui, Yunnan, and Beijing, fell into genogroup D, which was further divided into 3 subgenogroups, D1 D3, representing isolates from 1990 2003, 2002 2010, and 2004 2009, respectively.
Clusters 1 2 represent isolates from the first clade, whereas isolates from the second clade belong to clusters 3 7 and the solitaire isolate.
In this sub-clade are also represented isolates from Europe, Asia, North America and others African countries.
Colored bars represent isolates that are closely related based upon their Raman spectra.
Terminal nodes (tips) associated with the proximal partition of the tree represent isolates whose lineages are bound for extinction; the tips in the distal partition represent isolates descending from the breakpoint branch.
Following the guidelines on nanovirus species demarcation, this suggests that Mor23 and Mor5 represent isolates of FBNYV and FBNSV, respectively.
