Some of the genomic sequences used in this work were unfinished sequences and represent as yet unpublished material.
Together, these results indicate evolutionary conservation of gene interaction and suggest novel interactors from the Yor1-ΔF screen may represent as yet uncharacterized modifiers CFTR-ΔF biogenesis.
These include tags matching intergenic regions, which could represent as yet unannotated genes, transposable elements or small RNAs and tags that remain unassigned to the available genome sequence.
Taken together, these features indicate that identified contig clusters may, in many cases, represent as yet unannotated human lncRNAs or lncRNA fragments.
Finally, it is possible that these sequences represent as yet unrecognized nucleomorphs, which are generally characterized by rapid rates of substitution [ 33].
Our results suggest that many peculiar and sometimes counterintuitive properties of real biological systems may represent as yet unexplored by-products of the Darwinian evolution and do not necessarily implicate unknown biological mechanisms or selective factors.
Of them, 61,650,777 nt (64.9%) overlapped with human annotated transcripts (Ensembl gene annotation, version 64 [ 20]) covering 61% of all annotated exons, while the remaining 36,938,906 nt (35.1%) represented as yet unannotated portions of the human brain transcriptome.
Previous studies have shown that the majority of the novel transcripts located outside of annotated gene regions, both sense and antisense, may represent as yet unannotated extensions of known genes [ 31].
While many of the antisense transcripts found in our study represent as yet unannotated extensions of the neighboring genes, these extensions frequently stretch for hundreds of base pairs, traversing the entire length of the sense genes.
