( beta ) was the coefficient for any independent variable, αi represented unknown intercept for any entity (country) and ( {u}_{it} ) was the error term.
Four spots (8, 9, 13 and 15) were identified to be gliadin proteins, one spot (14) was found to be an avenin-like b precursor protein, and the remaining 4 spots (7, 10, 11 and 12) represented unknown proteins (Figure S3B, data not shown).
In addition, the other 71 (17%) unigenes represented unknown transcripts gene models (UG).
Over 55% of the sequenced fragments in each subtracted library represented unknown genes, of which one half were not previously described as cDNA sequences.
The model fitted was, where y was time to death, sex and family were fitted as fixed effects, animal and ID were random animal effects and mu represented unknown random residual effects.
However, 147 out of the total 166 Caenorhabditis matches represented unknown or hypothetical proteins and, similarly, 47 out of 78 H. glycines sequences were novel, i.e., they did not share similarity with other known sequences.
As a result, we identified 409 gene entries with two and more EST copies, and of these 205 represented known genes and 204 represented unknown genes (Table 1 and Additional data files 1 and 2).
The seven cDNA clones that mapped to Xl-PSIDs with average intensity values under four represented unknown sequences, the BMP4 gene, spondin 2 (extracellular matrix protein), and prolyl 4-hydroxylase, beta polypeptide.
