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In the case of real-time PCR the fluorescent signal of the most abundant amplicon will reach its threshold cycle (or CT value, used to quantify template copy numbers [ 6]) and plateau unaffected by the presence of any other less represented template.
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We suggest, therefore, that on the basis of our proposed criteria, the moa microsatellite dataset is of high fidelity despite representing template molecules of c. 600 to 5000 years of age [10].
Additionally, oligonucleotides that are able to self-extend by forming hairpin structures were introduced into the chip layout as a template-independent control for the primer extension reaction; they represent template and primer at the same time (for sequences see " Materials and methods").
Jeroen Dijsselbloem, the Dutchman who heads the Eurogroup of finance ministers, made it clear on March 25th that the Cypriot deal represented a template: if banks fail, creditors can expect to pay the whole bill.
As plenty different approaches to BCSs and CB have been proposed a large number of pseudonyms and acronyms have been dispersed across literature such that attempts to represented biometric template protection schemes in unified architectures have been made [217].
This allows the construction of catalogues of patterns (represented as templates) and meta-theorems for frameworks.
The Merrill deal also represents a template for future arrangements intended to unwind C.D.S.'s, Mr. Dinallo said.
Quite apart from the fact that the Springboks were giving run-outs to so many fringe players, the Barbarians hardly represent the template for how England play.
As such, his design represents a template for construction that can be replicated across the country.The team applies its plan to Oxford, a city with a large green belt where house prices are 11 times average earnings.
They may represent a template for the design of peptides with therapeutic applications as immunostimulatory agents.
More precisely, this pattern of activity may encode an intention and, thus, represent a "template for action".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com