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The remaining three insertions (2 SVA and 1 Alu) occurred in repetitive genomic regions and were detected by multiple unique reads in at least ten different samples each, indicating that these may have represented PCR false negatives.
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The Salmonella-specific microarray used in this study represented PCR-amplified sequences from the annotated open reading frames (ORFs) of S. Typhimurium LT2 and was further supplemented with annotated ORFs from the serovar Typhi CT18 strain that were > 10% divergent from those of serovar Typhimurium [ 60].
Data were transformed into a semi-log plot where the X-axis represents PCR cycle number and the Y-axis represents the log value of fluorescent intensity.
Input represents PCR amplification of DNA from cell lysate before immunoprecipitation with the primers used to amplify the p27kip1 promoter and β-actin (forward, 5'-CCA CAC TGT GCC CAT CTA CG-3'; reverse, 5'- AGG ATC TTC ATG AGG TAG TCA GTC AGTC').
Figure 5b represents PCR-amplified products of untreated and APDT-treated samples run on agarose gel electrophoresis.
For example, cDNAs that are not easily amplified by means of PCR are inadequately represented in PCR-based methods.
Grade 5 response of the primary tumour represented a pCR.
The most profound difference between the two techniques in detection of bacteria in the human gut, however, concerned the enumeration of high G+C Gram-positive bacteria such as Atopobium spp. and Bifidobacterium spp.: FISH technique consistently detected a sizable population of these genera in the gut while they were hardly represented in PCR-generated libraries.
Until recently, the technology represented by PCR-based determination of microsatellites only allowed a modest number of polymorphic markers to be used, which limited the resolution of the technique.
In the group of 26 resected patients, pCR was seen in 2 patients which represents a pCR rate of 7.4%95%5% CI 0 17) or 5.9%95%5% CI 0 14) in the intent-to-treat population, which met the criteria for early study termination due to lack of treatment efficacy based on pCR.
PCR fragments were tested both through 1% agarose gel, in which we had a clear solitary band representing the PCR product located between 700 and 500 DNA 100 bp ladder, and sequencing (The center for genomic technologies, Hebrew University, Jerusalem).
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