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Each gene was represented on the array as one spot containing immobilized cDNA fragments.
Of 27 APPG genes represented on the array, 26 were up regulated during one or more cuticular synthesis period.
Based on the annotation analysis, one or several different transcripts from 16644 ferret genes were represented on the array.
This approach demonstrated that only one soybean ortholog of an Arabidopsis iron uptake gene was represented on the array.
There are also probes corresponding to large intergenic regions from J2315 represented on the array.
A total of 1765 MGAS6180 genes were represented on the array.
Interestingly, approximately 90% of genes represented on the array exhibit no detectable variation between the examined seasons.
This means that for each gene represented on the array, there are between 160 and 240 measurements of expression level.
Microarrays were replicated 6 times (3 biological replicates per treatment, each with a dye swap) and each gene was represented on the array 3 to 7 times.
In general the orthopoxvirus strains represented on the array exhibit a high degree of protein sequence similarity, with ZAIRE and WRAIR sharing 62 identical proteins.
In the genome of the three strains used in this study only MMH594 contains the entire enterococcal PAI (129 genes, of which 125 were represented on the array).
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