Some of these transcripts were among the more represented ESTs in juveniles (Table 2).
Array positions with ≥0.25 mean log2 fold change ratio represented ESTs which were upregulated by the various treatments, and array positions with ≤ −0.25 mean log2 fold change ratio represented ESTs which were downregulated by the various treatments.
The unigenes with a redundancy level higher than 0.5% are listed in Table 3. Twenty most represented ESTs in zebrafish olfactory epithelium.
The unclassified category (99, 7.5% of total) represented ESTs for which a protein identity could be assigned based upon an alignment with known sequences, but the metabolic function of that sequence remained unknown.
Codon aligned orthologous sequence pairs were generated by removing gaps, ambiguous regions including runs of single amino acids with gaps, misidentified introns or likely sequencing errors within poorly represented ESTs.
aGenBank accession number for the representing EST of each gene.
In view of the high depth of coverage (8X) of our genomic DNA sequences, we believe that the non C-to-U changes represent EST sequencing errors.
We also designed 40-mer probes representing ESTs shorter than 60 bps.
As expected, TFM (floral meristem) library, representing ESTs from reproductive tissue showed unique expression that differentiated from all other tissue type libraries.
monococcum), a further 539 represent ESTs of the tetraploid (A and B genomes) macaroni wheat species T. turgidum, and five are from ESTs of a diploid near relative of the D genome known as Aegilops tauschii.
