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Public database sequences extending the 3' end of the expression data-derived sequences were included in the array design process along with the original expression data-derived sequences, since the latter were detected experimentally and likely represented alternatively polyadenylated or splice forms.
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These models were developed by employing a genetic algorithm with binary chromosome ring representing alternatively placed hot and cold layer fluid streams.
Using RT-PCR, we obtained products representing alternatively spliced PECAM-1 isoforms from mouse kidney total mRNA and generated PECAM-1-GST constructs expressing full length and naturally occurring alternatively spliced PECAM-1 variants.
The multiple bands in immunoblots of MDC1 (Figs. 2, 3 and 4) apparently represent alternatively spliced forms [19].
The sAC protein was initially purified from rat testis cytosol, and two independent cDNAs, which were subsequently shown to represent alternatively spliced isoforms [4], were cloned from a rat testis cDNA library [2].
bIndicates KOME cDNA accessions that may represent alternatively spliced isoforms.
These bands may represent alternatively spliced p53 isoforms as described in mammals [ 56].
These sets include contigs that may represent alternatively spliced forms of the same gene locus.
The remaining 6,228 isogroups were represented by multiple isotigs, which suggests they represent alternatively spliced transcripts from the same gene.
Finally, BCCs with contigs failing to meet the first two criteria were flagged as putatively representing alternatively spliced transcripts.
Each of these connected components does not contain cycles and should mostly represent alternatively spliced variants of one gene.
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