In this case, saturation occurred at curcuminoid concentrations of about 2000 μM, which represented a molar curcuminoid/BSA ratio of about 2.7.
These concentrations were more than twice as high as those achieved with FCS (Fig. 2A) and they represented a molar ratio of curcuminoid/BSA of about 1.6 1.9.
When the constant amount of 30 mg solid curcuminoids was added to 1 ml of solutions with increasing concentrations of BSA (0.2 10%), saturation was observed at about 5% BSA yielding a soluble curcuminoid concentration of about 1200 μM, which again represented a molar curcuminoid/BSA ratio of 1.6 (Fig. 3C).
For polyethylene, this represents a molar mass of 1800 g mol−1.
The best clone, 43RCA, had an IC50 equal to 23 ± 3 ng/ml, representing a molar ratio [43RCA]/[ricin] equal to 4. This clone was sufficiently expressed (around 100 μg/culture liter) to allow further study.
HSA (15 μM) (Abcam, Cambridge, MA) was incubated with increasing amounts of adenine propenal (0.15, 0.38, 1.5, 3, and 7 mM), representing a molar excess of adenine propenal to HSA of 10, 25, 100, 250, and 500, respectively.
SsoCas1 showed maximal activity at 250 nM, representing a fivefold molar excess of enzyme over substrate, with a decline in activity above 500 nM enzyme.
Upon digestion, TS oligo fragments can potentially represent a vast molar majority of the DNA fragments in the cDNA population, making downstream cloning and sequencing inefficient and more costly.
LACM 149371, an enigmatic tooth from the Paleogene of Santa Rosa, Peru, may represent an upper molar of an animal related to Ferugliotherium.
This represents an equal molar amount of plasmid DNA compared to the EGFP DNA contained in 5 ng total Aristolochia genomic DNA present in the leaf extract, assuming single copy/insertion of the EGFP gene.
For example, 2,2′,4-trichlorobiphenyl represented 4% (on a molar basis) of the total mixture before dechlorination and 16% of the dechlorinated product.
