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Representativity was assessed by histopathologic analysis from the immediate vicinity (neighbour section) to the biopsy used for extraction of total RNA.
In parallel, the extent of skeletal representativity was also in most cases considerably high again reaching between c. 80 and 100% of maximum values (between 95 and 126 points of maximum 126 points, average 89.2%).
The issues for external validity include that the sample size was relatively small and all patients came from Paris, France; however representativity was increased by recruitment from both tertiary care and private practices and the patient characteristics are in keeping with usual data for these populations.
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How this is done in practice, and with which consequences for representativity, is another research challenge that has to be addressed.
Representativity requirements are discussed for various wind data users.
Although no claims of representativity can be made based on the methods employed in this study, expressed views were relatively consistent between respondents, both within and between settings.
This implicit assumption of representativity (which is present in all simple imputation techniques) may be considered a weakness, and should be questioned when the treatment effect changes during follow-up.
However, due to the lack of numerical representativity it is not possible to make inferences about the relative or absolute incidence of the observed phenomena in the background population [ 33].
In the interest of representativity, the sample was weighted according to sociodemographic characteristics in order to represent the total Mayotte population and to account for potential oversampling and nonresponse.
To ensure representativity, the sample was stratified according to administrative district and type of institution.
It has to be mentioned that representativity for age groups was planned to be asured by the school grades representativity.
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