The trees were constructed using Muscle multiple sequence alignments for: a) human and Danio rerio P-DUDES domains, b) for the set of 145 representative P-DUDES domainsupplementeded by all Gallus gallus and D. rerio domains.
For example, informal feedback via community representatives and project fieldworkers, supplemented and validated by formal qualitative research methods [ 34], allowed misconceptions and rumours (uzushi) about the trial, its objectives and the risks or benefits of gel use to be captured, and locally-appropriate responses implemented.
According to Mr Straw, not only should the bishops stay, they should also be supplemented by representatives of other religions.
Taking into account the second specification, we supplemented the representatives selected on the basis of sequence with additional proteomes based on the Gene Ontology Reference Genome Project.
Selected sequences were supplemented with representatives of genotype 3e (strain G2, GenBank accession no. AF110389), 3f (strain G1, accession no. AF110388), and 3g (strain Osh 205, accession no. AF455784) (2 ).
These observations were supplemented with representative specimens captured using an anesthetic ejection system coupled to a suction hose that empties into a collecting chamber55.
All the patients who participated reported high-frequency headache, and were probably not representative, so their input was supplemented by interviews with two less-severely affected people with headache.
The MCP sequences of the PLV were supplemented with a representative sample of the "solitary" MCPs that were detected in our search of the metagenomic and genomic sequences, and subjected to phylogenetic analysis jointly with a selection of the Polinton MCPs that appear to be the closest homologs identified in database searches (see Additional files 3 and 4).
The validity of our taxonomic analysis was tested with two simulated datasets composed of 43 small or large subunit rRNAs from bacterial (32), archaeal (5) and eukaryotic (6) representatives (see Supplement Methods and Results S1 in SI for details).
The final search strategy was agreed following further discussion with all investigators, user and carer representatives (online supplement DS2).
