Detection of U. parvum and U. urealyticum with PCR primers targeting MBA genes with variable regions has been well documented for Ureaplasma detection [ 13, 32- 36] therefore, we selected this gene for PCR detection of Ureaplasma spp. In fact, as shown in Figure 1 (representative PCR results), each of the PCR products was clearly visible, permitting us to make accurate judgements.
Pharmaceutical company representatives (PCRs) influence the prescribing habits and professional behavior of physicians [ 1].
Pharmaceutical company representatives (PCRs) influence the prescribing habits and professional behaviour of physicians.
To verify that amplified products were derived from mRNA and did not represent genomic DNA contamination, representative PCR mixtures for each gene were run in the absence of the RT enzyme after first being cycled to 95°C for 15 min. In the absence of reverse transcription, no PCR products were observed.
Time-average equilibrium core was considered for the evaluation of the representative PCR of CANDU6.
Figure 6c is a representative PCR result, which shows that the adenocarcinoma shown in Figure 6b tested positive for eae, but that normal colonic mucosa from the same patient did not.
Representative PCR products were cloned and sequenced.
To control the false positive with either pair of primers, the representative PCR products, were T-cloned and sequenced.
Representative PCR products were purified by electrophoresis in 2% agarose gel followed by extraction from the gel by using a gel extraction kit (QIAGEN) according to the manufacturer's recommended instructions.
To verify the melting curve results, we analyzed representative PCR samples in 2.0 % agarose gels, purified and directly sequenced them from both directions by an automated DNA sequencer (ABI prism 310 Genetic Analyzer; Applied Biosystems, Foster City, CA, USA).
We then performed TR4 ChIP experiments for the other cell types and used standard PCR to confirm enrichment at three sites (TNFIAP1, SCAP, ECSIT) previously identified in HepG2 cells (see Additional file 2 for primer information; see Additional file 3 for representative PCR validation).
