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Results were parsed into a table (Additional file 1: Table S1) where a representative sequence was selected to represent every single protein family, each P. aeruginosa isolate has a column indicating the protein family representatives copy numbers within each of the analyzed genomes.
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aContig sequence containing a representative copy is listed.
The monomer with maximum length was used as the representative copy for the satellite family and as the query sequence in further BLAST searches.
These duplicates will unduly strengthen the case for a given genomic interaction and therefore HiCUP removes all but one representative copy of the di-tag.
The same analysis was done comparing the TE family representative sequences to the flax assembly, and a TE was considered as a representative copy of the TE family if it matched in 80% of its sequence to the query.
Representative CDO504 copy A and B sequences were chosen that covered the sequence diversity observed in this study.
If the truncated LTR copies were real vestiges of paralogous copies of families and if they had similar lengthes to the representative copies at the time of insertion, the difference of the length of truncated and representative copies provided the amount of deleted DNA since their insertion [ 36].
Larger sized genes, for which the representative copy-numbers were determined by averaging a greater number of probe copy-numbers within the gene, were used to cancel out noise in the gene copy-number [ 27].
Furthermore, a multiple alignment of representative copies, their coding capacity, and predicted protein secondary structure of the putative transposase, as well as paralogous empty sites are presented for each Spy family identified in these various species in supplementary figure S4, Supplementary Material online.
Majority-rule consensus sequences were reconstructed for an autonomous element (2,571 bp) encoding a 602-amino acid transposase and for a major family of non-autonomous elements (192 bp) based on an alignment of 25 representative copies of each type (Additional file 1).
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