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Results suggest that the proposed methodology provides an accurate and representative quantification of biological porosity and illuvial clay features in large thin sections.
The experiment was repeated three times with similar results and representative quantification of signals adjusted for background are shown in Figure 5. Genomic fragments were amplified by PCR for each cell cycle AP2 gene using a design that fuses the C-terminal end of each coding region with YFP in vector pYFP.LIC.DHFR [51].
A previous study that undertook behavioural observations on 10 fish over 20 consecutive 1 min periods found that a 3 min observation period was sufficient to obtain a representative quantification of behaviour for P. amboinensis (Mero and McCormick unpublished data; also see [38]).
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Representative images and quantification of GFP cluster size distribution in 1- to 5-month-old YapKIAlb-Cre mouse livers.
(B ) Representative immunoblot and quantification of the normalized OD (mean + SEM, Student's t test, n = 3, *p < 0.05).
Representative images and quantification of the BrdU+ hepatocytes of the 1.5-month-old YapCKOAlb-Cre and littermate control mice at steady state.
Figure 1 shows representative images and quantification of the optimal vessel formation achieved when co-culturing BMEC or HUVEC with either hBMSC or hDF at the optimal endothelial:stromal cell ratio.
Furthermore Notch-1 IC protein expression in HMVEC was significantly decreased in the presence of DAPT (see online supplementary figure S2D). Figure 4A,B shows representative images and quantification of HMVEC invasion demonstrating significant EC invasion in response to VEGF and Ang2 alone, which was further potentiated by VEGF/Ang2 combination.
Representative immunocytochemistry images and quantification of in vitro microglial proliferation (a,b) and phagocytosis of Aβ (c,d) with and without co-cultured NSCs.
(F-H) Representative 4× images and quantification of colonies formed by (F) low BST-2-expressing (G) medium BST-2-expressing, and (H) high BST-2-expressing human breast cancer cells that were treated with Vehicle or B49Mod1 (500 ng/well) every 3 days.
(A,B) Representative 4× images and quantification of spheres formed by (A) 4T1 cells, (B) E0771, (C) shCTL MDA-MB 231 and (D) shBST-2 MDA-MB 231 cells embedded in VitroGel 3D and treated with Vehicle or B49Mod1 (500 ng/well).
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