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This Kurt J. Lesker-built system is housed in the SMIF cleanroom facility here at Duke and some representative pictures are included below.
The decreased vascularization in treated tumors was further validated by immunostaining of corresponding tumor sections and subsequent quantification of CD31-positive area fractions (control group, 4.8 ± 0.50%; therapy group, 1.7 ± 0.37%; **P < 0.001; Figure 4D; representative pictures are shown in Figure 4E).
Representative pictures are presented on Fig. 6D for the MCF7 cells.
Representative pictures are presented.
Representative pictures are shown.
Representative pictures are shown in Figure 12.
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The fluorescent signals from stained cells were observed on an inverted fluorescent microscope (ApoTome fluorescence microscope, Zeiss) and representative pictures were taken.
Representative pictures were taken 24, 48 and 72 hr after infection.
All sections were counterstained with Harris's haematoxylin and representative pictures were taken using an Axiovision microscope.
For amylase and CK19 staining, at least 3 5 mice per time point and per genotype were stained and representative pictures were taken.
After 5 to 7 days, representative pictures were taken and spheroids were collected by filtration through a 40 µm mesh for the following experiments.
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