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This paper takes a practical approach – through the use of example – to clarifying what ontologies are and how they might be useful in an important and representative phase of the engineering design process, that of design requirement development and capture.
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We performed RNA sequencing analysis on weevil midguts at three representative phases of the endosymbiont dynamics (i.e. increase, climax and decrease).
The final model is shown in Figures 1 and 2. The statistics of the FE model are presented in Tables 1 and 2. Three FE models were made, one for each of the three most representative phases of gait cycle: heel strike, mid-stance and toe off.
Figure 1g shows a representative phase contrast image of the adherence of HTCD to the human colon epithelial host cells after three wash steps.
Compared to representative phase contrast images of PC12 cells not exposed to magnetic nanospheres and either (a) not treated or (b) treated daily with 100 ng/mL nerve growth factor (NGF), cells exposed to magnetic nanospheres with an iron concentration of 6.0 mM display similar morphology in the (c) absence or (d) presence of NGF.
(E ) Representative phase contrast images of spheres derived from Lu2 and Lu4 cell populations grown in the absence or presence of HGF for 10 days.
Left panels: Representative phase contrast images of HGF-treated and untreated spheres (bar, 400 μm) and H&E staining of sections through HGF-treated and untreated spheres (bar, 150 μm).
The idea is to take advantage of what a random perturbation of the representative phase point would do to the evolution of a system.
Examination of mRNA and protein levels of representative phase I and phase II enzymes validated the results obtained in our cell-based system.
Just before adding MTT reagent, representative phase contrast microscope images of cells were taken using an Olympus BX 41 microscope (Olympus, Center Valley, PA, USA).
In Fig. 6, the model nullclines are shown together with the bunch of the representative phase trajectories.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com