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Due to limited availability of antibodies and HepG2 cell lysate protein extract, validation was performed on the three selective proteins that are representative of separate mitochondrial functions and metabolic activities.
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In that study, we used four different contents, which had been identified in the first study as being representatives of separate content classes giving distinctively different results.
However, as several of the interventions, notably in Zanzibar, had been made through community groups the evaluation did team conduct four focus group discussions with representatives of separate communities at Shehia level.
Values represent the OD at 492 nm and are representative of two separate experiments (* P < 0.05 versus CTB-Fim2 IP, ** P < 0.001 and *** P < 0.0001 versus PBS, CTB, and rFim2 IN, ° P < 0.05 versus PBS and CTB, + P < 0.05 versus PBS, CTB, and rFim2 IN and IP, and ++ P < 0.001 versus CTB-Fim2 IP).
Images are representative of two separate experiments.
Data are representative of 3 separate experiments from 1 huPBMC donor.
Results are representative of two separate experiments.
The Ca2+ traces shown are representative of 4 separate experiments.
Unless otherwise stated, data presented are mean ± standard deviation of three replicate wells of a single prep of cells; the results shown are representative of 3 5 separate experiments each performed on different days and using a different preparation of cells.
Our analysis of ergosterol levels by previously described methods [12] demonstrated that AC had lower cell membrane ergosterol content when compared to PC (32.3% PC versus 10.1% AC for CLF 52; 37.6% PC versus 4.8% AC for CLF 29; p = 0.01; representative of three separate experiments).
Blots are representative of two separate experiments.
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