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Whenever possible, multiple representative ITS sequences from the GenBank for each of the closely related Russula species were also included to compare potential intra-specific variation of the Dahongjun phylogenetic groups to those within known Russula species.
One representative ITS sequence for each OTU was subsequently queried against GenBank using BLASTN [37], and whether one OTU was known or unknown was differentiated with the 97% sequence similarity criterion.
Representative ITS and 5.8S rRNA sequences were obtained from GenBank and initially aligned with Clustal X version 1.63b (Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg, France) and then by visual optimization.
"The commitments made under the Action Plan by the Government, the Army and the UN are being closely observed by the United Nations and the wider international community," said Mr. Ramesh Shrestha, UNICEF Myanmar Representative, "Its success on one hand it is a test for Myanmar.
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All further analyses of the parasite data were then based on the dataset including only representative ITS-sequence variants ("TCS-types").
The PCA was calculated in SPSS 20.0 (using varimax rotation) and was based on the frequencies of Caullerya representative ITS-sequence variants (TCS-types) in each sample.
Calculations were based on the frequency of representative ITS-sequence variants (TCS-types) in C. mesnili parasite DNA (10 individually genotyped Daphnia hosts per sample; for selection of samples see Table 1).
Both ITS data sets (obtained by DNA extraction from pooled or single hosts, see Table 1) have recently been used for validation of DNA pooling and for comparison of different statistical methods identifying representative ITS-sequence variants (i.e., statistical parsimony networks vs. neighbour-joining analysis, [ 40]).
To study whether the visits of technical health representatives (ITS) mean that new drugs are introduced.
Each preliminary cluster is initialized by taking its representative as its initial member.
The student representatives, its most powerless members, are picked by the Chancellor's High School Student Advisory Council, which is made up of students representing high schools citywide.
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