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Representative fruits were ground with a GM 200 (Grindomix) mill to pass a 20 screen mesh, not more than a week prior to capsaicinoid isolation.
At each developmental stage, ten representative fruits were sampled from each tree.
Three different trees were used, and 10 representative fruits from each tree were collected.
As an example of the variability among fruits within the mapping population, pictures of several representative fruits grown at EJ are shown in Additional file 3: Figure S2.
With this aim, six representative fruits from each tree were collected at harvest, and stored at 4 °C until the analysis.
For molecular and biochemical analyses, two replicates of four representative fruits of each stage were sampled, peeled, cut into 0.5-cm slices and the mesocarp was immediately frozen in liquid nitrogen and stored at -80 °C.
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We found that gaseous CO2 fertilizers enhanced the yield of representative fruit vegetable (i.e., cucumber, tomato, chili, zucchini, eggplant, and strawberry) by 33.31% over the past three decades.
Thai consumers were also asked to evaluate seven representative fruit leather products using a 9-point hedonic scale for various sensory attributes as well as overall liking and their responses together with data that was obtained from a QDA panel was used in the QFD to define the importance of the various sensory characteristics for the design of a new fruit leather.
Apples were thinned to a low crop load of 4 fruit per cm2 of trunk size and a minimum of five representative fruit sizes were weighed at harvest.
When the 'Royal Gala' control fruit began to show a change in background skin colour, and representative fruit had a starch index of 1 2 (0–7 scale), all fruit were harvested for both genotypes.
Ten representative fruit per tree were harvested and for five of these fruit, fresh weight was recorded and maximum firmness measured using a GUSS penetrometer with an 11.1-mm probe inserted at a speed of 10 mm per second.
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