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Here, we present an integrated approach through a systematic model-driven evaluation of the production potential for the bacterial production organism Escherichia coli to produce multiple native products from different representative feedstocks through coupling metabolite production to growth rate.
First, we investigated the effect of pretreatment temperature on carbohydrate release for four representative feedstocks (corn stover, switchgrass, sorghum, and poplar); this shows the trade-offs associated with the development of a screening assay, which (by definition) is performed with a specific set of conditions.
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Representative feedstock and raw biochar are shown in Figure 1.
The chemical composition of the representative feedstock was as follows: 36.3% glucan, 21.4% xylan, 21.3% Klason lignin.
Glucose was chosen as a representative sugar feedstock as it is frequently provided in R. opacus cell cultures3,9,27.
We first present pretreatment and enzymatic hydrolysis experiments on a set of representative biomass feedstock samples (corn stover, poplar, sorghum, switchgrass) in order to put the assay in context, and then show the results of the assay applied to approximately 150 different feedstock samples covering 5 different materials.
Xylan was chosen as a model compound for hemicelluloses, and two kinds of agricultural wastes rice straw and palm shell were used as typical feedstocks representative of herbaceous and woody biomasses, respectively.
The reference data set was composed of the 'unique' plants established from the PCA metrics, and a random selection of feedstocks representative of the majority of the samples.
Routes were simulated using dedicated industrial process simulation software, taking as feedstock a representative and robust modeled composition of Chlorella sp. microalgae biomass.
Comparison of activities indicates DBT as more representative of a real feedstock and the CoMo/TiO2 (50% -Al2O3 (50% -Al2O3yst appears more active than the CoMo/Al2O3 sample toward HDS, HDN and hydrodearomatization.
Therefore, our initial approach was to select a broad range of lignocellulosic feedstocks, including representatives of agricultural and forest biomass, and to pretreat these materials under conditions that allowed maximum hemicellulose recovery and good enzymatic hydrolysis of the cellulosic component.
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CEO of Professional Science Editing for Scientists @ prosciediting.com