Exact(60)
These usually share the same chemical representation library, but full alignment between them is not always certain.
Using only the 2 3 Kb fragments resulted in a "reduced representation" library [52].
Samples were quantified by spectrophotometry, and at least 300 ng of purified DNA was used to construct a RRL (reduced representation library) for sequencing.
Using a novel reduced representation library (RRL) next-generation sequencing strategy to discover large numbers of genome-wide SNP with high MAF [16].
We generated a reduced representation library (RRL) [25] to reduce the complexity of the white-tailed deer genome and a random shotgun library (RSL) to enable massively parallel pyrosequencing via the Roche 454 platform.
To achieve better consistency, these contigs were renamed in the format of "GMAGB2RR000001" with "GM" standing for genomic, "AG" for Aphis glycines, "B2" for B2, "RR" for reduced representation library, and "000001" for an arbitrarily assigned number.
We utilized massively parallel pyrosequencing of a reduced representation library (RRL) and a random shotgun library (RSL) to generate a complete mitochondrial genome sequence and identify a large number of putative single nucleotide polymorphisms (SNPs) distributed throughout the white-tailed deer nuclear and mitochondrial genomes.
CR created the reduced representation library, and assisted the manuscript.
Reduced representation library construction and Illumina sequencing yielded 230,232,173 (100 bp) reads.
The method to construct the reduced representation library is described elsewhere [ 10].
The isolated reduced representation library (RRL) was paired-end sequenced using Illumina genome Analyzer technology.
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